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The inactivated cooled reaction mixture was added to 50 μl NAD + , 10 μl of D-lactate dehydrogenase and 20 mM sodium phosphate buffer (pH 7.6) in a reaction mixture of 1000 μl followed by incubation for atleast 10 min at room temperature or longer until the reaction reaches the endpoint (absorbance does not vary further)

European standards

Flavor:BERRY BLAST

The next day, sections were incubated with secondary antibodies for 1 h and visualized using DAB staining (Maixin Biotechnology Development Co., Ltd., Fuzhou, China)