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2.3 Immunological challenge and oxidative metabolism parameters An immunological challenge was performed on all calves on day 40 of the experiment and inoculation was carried out by applying 1 mL via intramuscularly puncture of low-virulence isolates of Anaplasma marginale (1x10 7 red blood cells infected, registered in GenBank under codes EU676176 (CEPA UFMG1)
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